The archaeal DnaG protein needs Csl4 for binding to the exosome and enhances its interaction with adenine-rich RNAs.
作者： Linlin Hou , Gabriele Klug , Elena Evguenieva-Hackenberg
DOI: 10.4161/RNA.23450
关键词:
摘要: The archaeal RNA-degrading exosome contains a catalytically active hexameric core, an RNA-binding cap formed by Rrp4 and Csl4 the protein annotated as DnaG (bacterial type primase) with so-far-unknown functions in RNA metabolism. We found that binds to Csl4-exosome but not Rrp4-exosome of Sulfolobus solfataricus. In vitro assays revealed is poly(A)-binding enhancing degradation adenine-rich transcripts Csl4-exosome. second besides heteromeric, S. solfataricus exosome. This apparently reflects need for effective selective recruitment RNAs metabolism
researchgate.net 
sci-hub.se 参考文章(30)
Esben Lorentzen, Elena Conti, Expression, reconstitution, and structure of an archaeal RNA degrading exosome Methods in Enzymology. ,vol. 447, pp. 417- 435 ,(2008) , 10.1016/S0076-6879(08)02220-9
B. K. Mohanty, S. R. Kushner, Polynucleotide phosphorylase functions both as a 3′ → 5′ exonuclease and a poly(A) polymerase in Escherichia coli Proceedings of the National Academy of Sciences of the United States of America. ,vol. 97, pp. 11966- 11971 ,(2000) , 10.1073/PNAS.220295997
Emmanuele De Vendittis, Vincenzo Bocchini, Protein-encoding genes in the sulfothermophilic archaea Sulfolobus and Pyrococcus Gene. ,vol. 176, pp. 27- 33 ,(1996) , 10.1016/0378-1119(96)00203-X
Anna Oddone, Esben Lorentzen, Jerome Basquin, Alexander Gasch, Vladimir Rybin, Elena Conti, Michael Sattler, Structural and Biochemical Characterization of the Yeast Exosome Component Rrp40 EMBO Reports. ,vol. 8, pp. 63- 69 ,(2007) , 10.1038/SJ.EMBOR.7400856
Marcos V. A. S. Navarro, Carla C. Oliveira, Nilson I. T. Zanchin, Beatriz G. Guimarães, Insights into the mechanism of progressive RNA degradation by the archaeal exosome. Journal of Biological Chemistry. ,vol. 283, pp. 14120- 14131 ,(2008) , 10.1074/JBC.M801005200
Elena Evguenieva‐Hackenberg, Pamela Walter, Elisabeth Hochleitner, Friedrich Lottspeich, Gabriele Klug, An Exosome-Like Complex in Sulfolobus Solfataricus EMBO Reports. ,vol. 4, pp. 889- 893 ,(2003) , 10.1038/SJ.EMBOR.EMBOR929
Eugene V Koonin, Yuri I Wolf, L Aravind, Prediction of the Archaeal Exosome and Its Connections with the Proteasome and the Translation and Transcription Machineries by a Comparative-Genomic Approach Genome Research. ,vol. 11, pp. 240- 252 ,(2001) , 10.1101/GR.162001
Christian R. Eckmann, Christiane Rammelt, Elmar Wahle, Control of poly(A) tail length. Wiley Interdisciplinary Reviews - Rna. ,vol. 2, pp. 348- 361 ,(2011) , 10.1002/WRNA.56
Elena Evguenieva-Hackenberg, Verena Roppelt, Pamela Finsterseifer, Gabriele Klug, Rrp4 and Csl4 are needed for efficient degradation but not for polyadenylation of synthetic and natural RNA by the archaeal exosome. Biochemistry. ,vol. 47, pp. 13158- 13168 ,(2008) , 10.1021/BI8012214
Verena Roppelt, Gabriele Klug, Elena Evguenieva-Hackenberg, The evolutionarily conserved subunits Rrp4 and Csl4 confer different substrate specificities to the archaeal exosome FEBS Letters. ,vol. 584, pp. 2931- 2936 ,(2010) , 10.1016/J.FEBSLET.2010.05.014