Screening ethnically diverse human embryonic stem cells identifies a chromosome 20 minimal amplicon conferring growth advantage
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DOI: 10.1038/NBT.2051
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摘要: The International Stem Cell Initiative analyzed 125 human embryonic stem (ES) cell lines and 11 induced pluripotent (iPS) lines, from 38 laboratories worldwide, for genetic changes occurring during culture. Most were at an early late passage. Single-nucleotide polymorphism (SNP) analysis revealed that they included representatives of most major ethnic groups. remained karyotypically normal, but there was a progressive tendency to acquire on prolonged culture, commonly affecting chromosomes 1, 12, 17 20. DNA methylation patterns changed haphazardly with no link time in Structural variants, determined the SNP arrays, also appeared sporadically. No common variants related culture observed 12 17, minimal amplicon chromosome 20q11.21, including three genes expressed ES cells, ID1, BCL2L1 HM13, occurred >20% lines. Of these genes, is strong candidate driving adaptation cells.
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