Assessment of hypothalamic-pituitary-adrenal axis and sympathetic nervous system activity in pregnant sows through the measurement of glucocorticoids and catecholamines in urine.

作者: M Hay , M C Meunier-Salaün , F Brulaud , M Monnier , P Mormède

DOI: 10.2527/2000.782420X

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摘要: We validated the use of urine to monitor changes in activity both hypothalamic-pituitary-adrenal (HPA) axis and sympathetic nervous system (SNS) swine. Ten pregnant sows were fitted with venous catheters 3 wk after mating. In early (wk 6), middle 9), late 14) stages gestation, blood collected over 24 h diurnal plasma cortisol, urinary catecholamines (norepinephrine [NE] epinephrine [EPI]). Dexamethasone suppression tests (DST) ovine corticotropin-releasing hormone (CRH) challenge also performed at each stage gestation. All values changed markedly around clock. Diurnal variations cortisol comparable those plasma, a nocturnal peak trough occurring evening. During dark period, lower than during light period. Norepinephrine increased sharply lights came on peaked meal time. Epinephrine began rise end period just before Average due higher levels daylight hours. 2000 (20 microg/kg i.v.) decreased 0830 excretion. The magnitude decrease ACTH DST was midgestation, indicating feedback resistance that stage. CRH (1 cortisol. Peak occurred 30 min 2 injection, respectively. Catecholamines produced night (2000 0800) morning (0400 0800 0900) highly correlated their 24-h excretion rate. These results indicate it is possible HPA SNS through measurements pigs.

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