Sensitive and specific quantification of human genomic deoxyribonucleic acid (DNA) in forensic science specimens: casework examples.
作者： John S. Waye , Denis Michaud , John H. Bowen , Ron M. Fourney
DOI: 10.1520/JFS13136J
关键词:
摘要: We describe the forensic science application of a method for quantification human genomic deoxyribonucleic acid (DNA). The two cases cited in this report involve DNA samples extracted from skin tissue and bloodstained clothing recovered different crime scenes. High-molecular-weight was both specimens, concentrations these DNAs were estimated to be ∼0.5 µg/µL by ethidium bromide/agarose gel electrophoresis. Using human-specific probe p17H8 (locus D17Z1) quantify amount samples, it is shown that less than 1% isolated origin effectively devoid sequences. These case examples illustrate need not only total casework material, but also proportion origin.
sci-hub.st 参考文章(9)
Bruce Budowle, R. M. Fourney, L. A. Presley, J. S. Waye, G. G. Shutler, A simple and sensitive method for quantifying human genomic DNA in forensic specimen extracts BioTechniques. ,vol. 7, pp. 852- 855 ,(1989)
M Clyne, E Meade, I Balazs, M Baird, Human population genetic studies of five hypervariable DNA loci. American Journal of Human Genetics. ,vol. 44, pp. 182- 190 ,(1989)
Bruce Budowle, John S. Waye, Gary G. Shutler, F. Samuel Baechtel, Hae III--a suitable restriction endonuclease for restriction fragment length polymorphism analysis of biological evidence samples. Journal of Forensic Sciences. ,vol. 35, pp. 530- 536 ,(1990) , 10.1520/JFS12858J
J S Waye, S B England, H F Willard, Genomic organization of alpha satellite DNA on human chromosome 7: evidence for two distinct alphoid domains on a single chromosome. Molecular and Cellular Biology. ,vol. 7, pp. 349- 356 ,(1987) , 10.1128/MCB.7.1.349
J S Waye, H F Willard, Structure, organization, and sequence of alpha satellite DNA from human chromosome 17: evidence for evolution by unequal crossing-over and an ancestral pentamer repeat shared with the human X chromosome. Molecular and Cellular Biology. ,vol. 6, pp. 3156- 3165 ,(1986) , 10.1128/MCB.6.9.3156
Andrew P. Feinberg, Bert Vogelstein, A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity Analytical Biochemistry. ,vol. 132, pp. 6- 13 ,(1983) , 10.1016/0003-2697(83)90418-9
E.M. Southern, Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology. ,vol. 98, pp. 503- 517 ,(1975) , 10.1016/S0022-2836(75)80083-0
Y Nakamura, M Leppert, P O'Connell, R Wolff, T Holm, M Culver, C Martin, E Fujimoto, M Hoff, E Kumlin, al. et, Variable number of tandem repeat (VNTR) markers for human gene mapping Science. ,vol. 235, pp. 1616- 1622 ,(1987) , 10.1126/SCIENCE.3029872
Phillip A. Sharp, Bill Sugden, Joe Sambrook, Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis Biochemistry. ,vol. 12, pp. 3055- 3063 ,(1973) , 10.1021/BI00740A018