Evaluation of the AmpliType® PM DNA Test System on Forensic Case Samples

作者: George Herrin , Nicola Fildes , Rebecca Reynolds

DOI: 10.1520/JFS13710J

关键词: Sexual assaultMedical physicsGeneticsBiologyMolecular systemsForensic scienceDna testPathology and Forensic Medicine

摘要: Evidence material from sexual assault cases which had been submitted to the laboratory for routine processing were examined determine usefulness of AmpliType® PM PCR Amplification and Typing Kit developed by Roche Molecular Systems forensic evidence. In all in a conclusive answer was reached AmpliType system, results agreed with or surpassed previously obtained RFLP testing. The DNA test system has promise as quick easy method elimination inclusion suspects.

参考文章(17)
F. S. Baechtel, Bruce Budowle, Modifications to improve the effectiveness of restriction fragment length polymorphism typing. Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society. ,vol. 1, pp. 181- 187 ,(1990)
Alan Giusti, Michael Baird, Sam Pasquale, Ivan Balazs, Jeffrey Glassberg, Application of deoxyribonucleic acid (DNA) polymorphisms to the analysis of DNA recovered from sperm. Journal of Forensic Sciences. ,vol. 31, pp. 409- 417 ,(1986) , 10.1520/JFS12270J
Bruce Budowle, John S. Waye, Gary G. Shutler, F. Samuel Baechtel, Hae III--a suitable restriction endonuclease for restriction fragment length polymorphism analysis of biological evidence samples. Journal of Forensic Sciences. ,vol. 35, pp. 530- 536 ,(1990) , 10.1520/JFS12858J
John S. Waye, Denis Michaud, John H. Bowen, Ron M. Fourney, Sensitive and specific quantification of human genomic deoxyribonucleic acid (DNA) in forensic science specimens: casework examples. Journal of Forensic Sciences. ,vol. 36, pp. 1198- 1203 ,(1991) , 10.1520/JFS13136J
Edward Blake, Jennifer Mihalovich, Russell Higuchi, P. Sean Walsh, Henry Erlich, Polymerase Chain Reaction (PCR) Amplification and Human Leukocyte Antigen (HLA)-DQα Oligonucleotide Typing on Biological Evidence Samples: Casework Experience Journal of Forensic Sciences. ,vol. 37, pp. 700- 726 ,(1992) , 10.1520/JFS11984J
Catherine Theisen Comey, Bruce Budowle, Dwight E. Adams, Anne L. Baumstark, Jenifer A. Lindsey, Lawrence A. Presley, PCR amplification and typing of the HLA DQ alpha gene in forensic samples Journal of Forensic Sciences. ,vol. 38, pp. 239- 249 ,(1993) , 10.1520/JFS13402J
Catherine Theisen Comey, Bruce Budowle, Validation Studies on the Analysis of the HLA DQα Locus Using the Polymerase Chain Reaction Journal of Forensic Sciences. ,vol. 36, pp. 1633- 1648 ,(1991) , 10.1520/JFS13188J
William Klitz, Mark Stoneking, Rhea Helmuth, Norma Schmill, C. Gorodezky, Henry A. Erlich, Russell Higuchi, Michael C. Luce, Nicola Fildes, J. Chimera, Edward Blake, Roberta Madej, HLA-DQα allele and genotype frequencies in various human populations determined by using enzymatic amplification and oligonucleotide probes American Journal of Human Genetics. ,vol. 47, pp. 515- 523 ,(1990)
G T Horn, B Richards, J J Merrill, K W Klinger, Characterization and rapid diagnostic analysis of DNA polymorphisms closely linked to the cystic fibrosis locus. Clinical Chemistry. ,vol. 36, pp. 1614- 1619 ,(1990) , 10.1093/CLINCHEM/36.9.1614
R. K. Saiki, P. S. Walsh, C. H. Levenson, H. A. Erlich, Genetic analysis of amplified DNA with immobilized sequence-specific oligonucleotide probes Proceedings of the National Academy of Sciences of the United States of America. ,vol. 86, pp. 6230- 6234 ,(1989) , 10.1073/PNAS.86.16.6230