PCR amplification and typing of the HLA DQ alpha gene in forensic samples

摘要: The polymerase chain reaction (PCR) was used to amplify the HLA DQ alpha gene using DNA recovered from evidentiary samples. Amplified then typed sequence-specific oligonucleotide probes. Slight modifications of previously published extraction methods improved typing success bloodstains and semen-containing material. Evidentiary samples, consisting 206 known bloodstains, 26 questioned 123 materials were analyzed 96 cases by restriction fragment length polymorphism (RFLP) in FBI Laboratory. Of 98.5% yielded results. 102 149 (24/26 78/123 materials), or 68%, produced 78 that RFLP inclusions, 59 interpretable results these all inclusions. remaining 19 could not be interpreted for alpha. 18 exclusions, eleven four three It is expected because difference discrimination potential two methods, some exclusions would Some samples failed produce may have had insufficient analysis. Employment a human quantification method casework allow user more consistently use sufficient quantities amplification. also provide guide determining if an inhibitor PCR present, thus suggesting procedure improve This study provides support valid forensic