Sequential G-banding FISH on human sperm chromosomes
作者： P. Colls , C. Templado , O. MartUnez-Pasarell , F. Darroudi , A. T. Natarajan
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摘要: A method that allows the performance of double-colour chromosome painting (FISH) on previously G-banded human sperm metaphases has been developed. Sperm chromosomes were obtained by using fusion technique between zona-free hamster oocytes and spermatozoa. Single- was performed DNA libraries specific for X, Y 21 either unstained or preparations. The hybridization efficiency very high (98%). sequential staining is useful analyses structural (stable) numerical aberrations in thus can increase sperm-hamster system to assess risk germ cells as a result endogenous exogenous factors.
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sci-hub.se 参考文章(26)
K Burns, W Balkan, C C Lin, R H Martin, Direct chromosomal analysis of human spermatozoa: preliminary results from 18 normal men. American Journal of Human Genetics. ,vol. 34, pp. 459- 468 ,(1982)
R H Martin, E Ko, E Spriggs, A W Rademaker, The relationship between paternal age, sex ratios, and aneuploidy frequencies in human sperm, as assessed by multicolor FISH American Journal of Human Genetics. ,vol. 57, pp. 1395- 1399 ,(1995)
S. H Roberts, The Cytogenetics of Mammalian Autosomal Rearrangements Journal of Medical Genetics. ,vol. 26, pp. 415- 416 ,(1989) , 10.1136/JMG.26.6.415
A.K. Tarkowski, An Air-Drying Method for Chromosome Preparations from Mouse Eggs Cytogenetic and Genome Research. ,vol. 5, pp. 394- 400 ,(1966) , 10.1159/000129914
V.R. Babu, A. Wiktor, A fluorescence in situ hybridization technique for retrospective cytogenetic analysis. Cytogenetic and Genome Research. ,vol. 57, pp. 16- 17 ,(1991) , 10.1159/000133104
V.T.H.B.M. Smit, J.W.W. Wessels, P. Mollevanger, P.I. Schrier, A.K. Raap, G.C. Beverstock, C.J. Cornelisse, Combined GTG-banding and nonradioactive in situ hybridization improves characterization of complex karyotypes. Cytogenetic and Genome Research. ,vol. 54, pp. 20- 23 ,(1990) , 10.1159/000132947
V. T. H. B. M. Smit, J. W. Wessels, P. Mollevanger, J. G. Dauwerse, M. Van Vliet, G. C. Beverstock, M. H. Breuning, P. Devilee, A. K. Raap, C. J. Cornelisse, Improved interpretation of complex chromosomal rearrangements by combined GTG banding and in situ suppression hybridization using chromosome-specific libraries and cosmid probes. Genes, Chromosomes and Cancer. ,vol. 3, pp. 239- 248 ,(1991) , 10.1002/GCC.2870030402
B. Brandriff, L. Gordon, L. Ashworth, G. Watchmaker, A. Carrano, A. Wyrobek, Chromosomal abnormalities in human sperm: Comparisons among four healthy men Human Genetics. ,vol. 66, pp. 193- 201 ,(1984) , 10.1007/BF00286600
Renee H. Martin, Kathy Hildebrand, Judy Yamamoto, Alfred Rademaker, Michael Barnes, Gary Douglas, Keith Arthur, Thomas Ringrose, Ian S. Brown, An increased frequency of human sperm chromosomal abnormalities after radiotherapy Mutation Research Letters. ,vol. 174, pp. 219- 225 ,(1986) , 10.1016/0165-7992(86)90155-7
L. Tusell, R. Alvarez, M. R. Caballin, A. Genescà, R. Miró, M. Ribas, J. Egozcue, Induction of micronuclei in human sperm-hamster egg hybrids at the two-cell stage after in vitro gamma-irradiation of human spermatozoa. Environmental and Molecular Mutagenesis. ,vol. 26, pp. 315- 323 ,(1995) , 10.1002/EM.2850260407