Drug depot-anchoring hydrogel: A self-assembling scaffold for localized drug release and enhanced stem cell differentiation.

作者: Ruixiang Li , Zhiqing Pang , Huining He , Seungjin Lee , Jing Qin

DOI: 10.1016/J.JCONREL.2017.07.008

关键词:

摘要: Localized and long-term delivery of growth factors has been a long-standing challenge for stem cell-based tissue engineering. In the current study, polymeric drug depot-anchoring hydrogel scaffold was developed sustained release macromolecules to enhance differentiation cells. Self-assembling peptide (RADA16)-modified depots (RDDs) were prepared anchored RADA16 hydrogel. The anchoring effect modification on RDDs tested both in vitro vivo. It shown that leakage from significantly less than unmodified (DDs). addition, vivo retention injected hydrogel-incorporated longer DDs. A model drug, vascular endothelial factor (VEGF), encapsulated (V-RDDs) as depot then VEGF could be 4weeks. Endothelial progenitor cells (EPCs) cultured V-RDDs-anchoring enhanced cell proliferation observed, compared with VEGF-loaded scaffold. Furthermore, this laden EPCs promoted neovascularization an animal hind limb ischemia. These results demonstrate self-assembling hydrogel-anchored drug-loaded are promising localized release, can effectively resident cells, thus lead successful regeneration.

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