The presentation of class I and class II epitopes of listeriolysin O is regulated by intracellular localization and by intercellular spread of Listeria monocytogenes.

摘要: The hemolysin, listeriolysin 0 (LLO), produced by Listeria monocytogenes is both a virulence factor and an immunodominant Ag. In this study, we investigated how the lytic activity of LLO effects context presentation two known epitopes either class I or II MHC molecules. T cell hybridomas were used to monitor each peptide/MHC ligand. APCs infected with strains expressing hemolytic strongly presented epitope; however, ligand was not well cells nonhemolytic Listeria. contrast, there almost no II-binding epitope in fully Only hemolysin-deficient MHC. wild-type but deficient other factors showed pattern equivalent that To address further divergence presentation, intercellular spread assay detect Ag neighboring primarily one. We found on adjacent initially one(s). Finally, our kinetic analysis revealed over 4 h before have demonstrated LLO's potentiates listerial Ags inhibits via LLO-mediated intracellular localization (cytoplasmic vs endosomal) bacteria corresponds operative pathway.