Pseudomonas putida NBRIC19 dihydrolipoamide succinyltransferase (SucB) gene controls degradation of toxic allelochemicals produced by Parthenium hysterophorus

摘要: Aims:  The aim of this study was to identify the gene responsible for degradation toxic allelochemicals Parthenium by generating Tn5-induced mutant Pseudomonas putida NBRIC19. Furthermore, characterizes at physiological, biochemical and molecular level that helped in understanding mechanisms reducing allelopathic inhibition Ps. putida NBRIC19. Methods Results:  Tn5 S-74.3 showing inability degrade selected after screening 22 000 transconjugants. flanking SucB (dihydrolipoamide succinyltransferase) NBRIC19 found be also affected biofilm formation, chemotaxis alginate production under environment allelochemicals. Phenotypic microarray data revealed respiratory activity differed on 47 substrates including amino acids, carboxylic peptides some chemical inhibitors. Conclusions:  Study regulates processes either directly or indirectly NBRIC19, which inactivation made less compatible tolerating stress. Significance Impact Study:  This work provides first evidence a functional role lead reversal plant growth these investigation interesting features about involvement microbes plant–plant interactions.