Miniprep DNA isolation from unicellular and filamentous cyanobacteria
作者： Marli F Fiore , David H Moon , Siu M Tsai , Hung Lee , Jack T Trevors
DOI: 10.1016/S0167-7012(99)00110-4
关键词:
摘要: Abstract A rapid miniprep method for isolation of DNA from 12 strains cyanobacteria belonging to groups I, III, IV and V is described. The protocol a modification the methods Boyle Lew [Boyle, J.S., Lew, A.M., 1995. An inexpensive alternative glassmilk purification. Trends Genet. 11, 8] cetyltrimethyl ammonium bromide (CTAB) extraction Sahgai-Maroof et al. [Sahgai-Maroof, M.A., Soliman, K.M., Jorgensen, R.A., Allard, R.W., 1984. Ribosomal spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location population dynamics. Proc. Natl. Acad. Sci. USA 81, 8014–8018]. new especially useful obtaining cyanobacterial unicellular, filamentous branched species. does not require phenol product can be used directly PCR amplification restriction digestion.
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