Total body irradiation of donors can alter the course of tolerance and induce acute rejection in a spontaneous tolerance rat liver transplantation model

摘要: Liver transplantation is an established therapy for end-stage liver diseases. Graft rejection occurs unless the recipient receives immunosuppression after transplantation. This study aimed to explore mechanism of acute allografts in rats pre-treated with total body irradiation eliminate passenger lymphocytes and define role CD4+CD25+ regulatory T cells induction immunotolerance recipient. Male Lewis were used as donors male DA recipients. Rats randomly assigned following four groups: control group, homogeneity idio-immunotolerance group group. After transplantation, survival time each serum alanine aminotransferase, bilirubin levels, number Foxp3+CD4+CD25+ cells, expression glucocorticoid-induced tumor necrosis factor receptor on cell subgroups, histopathology hepatic graft spleen cytotoxic lymphocyte lytic activity measured. In where preconditioned before all recipients died between day 17 21. On 14, aminotransferase increased significantly (459.2±76.9) U L−1, (124.1±33.7) μmol L−1 (P<0.05) ratio decreased 1.50%±0.50% compared other groups. Analysis subpopulations varied from Histological analysis showed typical changes only. Preconditioning eliminated graft, thus affected course tolerance induced