Detection of endotoxin in plasma of hospitalized diarrheic calves

摘要: OBJECTIVES To investigate whether lipopolysaccharide (LPS) is present in plasma of calves with naturally occurring diarrhea. The second objective was to determine [LPS] correlates clinical, hematological, biochemical, and acid-base variables, differs between surviving nonsurviving diarrheic calves. DESIGN Prospective observational study (January 2012-May 2014). SETTING Veterinary teaching hospital. ANIMALS Thirty-four <28 days old admitted for diagnosis treatment diarrhea 30 healthy control MEASUREMENTS AND MAIN RESULTS Admission demographics, physical examination, blood gas, biochemistry analysis, outcome data were recorded. Plasma concentration LPS determined using a bovine ELISA assay. detected both significantly higher than (median: 0.99 ng/mL; Interquartile range (IQR): 0.068, vs 0.88 ng/mL; 0.065 ng/mL, respectively; P < 0.001). (1.04 ng/mL; 0.07 ng/mL) (0.98 ng/mL; 0.022 ng/mL; beef (1.07 ng/mL; 0.182 ng/mL) dairy (0.99 ng/mL; In calves, correlated [l-lactate] (r2 = 0.496; 0.002); hypoglycemia -0.453; 0.007); increased unmeasured strong ions 0.332; 0.050), [Mg2+ ] 0.475; 0.004), [phosphate] 0.468; 0.005), aspartate aminotransferase activity 0.348; 0.003). CONCLUSIONS This highlights potential role the pathogenesis metabolic derangements such as hyperlactatemia, hypoglycemia, anions Further investigation evaluating effect on l-lactate glucose metabolism warranted.