Chapter 9 Endothelial Adhesive Platforms Organize Receptors to Promote Leukocyte Extravasation

摘要: Publisher Summary Endothelium does not constitute merely a physical barrier but plays an active role during leukocyte transendothelial migration. Its contribution is based on the combined action of different adhesion receptors involved in subsequent steps process that are assisted by membrane organizers and cytoskeletal-associated components. In this regard, endothelial congregate constitutive submicrometer-sized clusters termed “endothelial adhesive platforms” (EAPs), instead being randomly distributed throughout apical plasma membrane. The organizing units such platforms tetraspanin proteins, which interact among themselves simultaneously with broad variety molecules, providing adequate receptor avidity to overcome threshold required for leukocyte–endothelium interactions occurring under shear flow. Therefore, these act as supramolecular mechanism spatiotemporally organize similar characteristics functions at facilitate their efficient coordinated extravasation, requires rapid kinetics. This chapter describes specific tetraspanin-enriched microdomains (TEMs) comparison other types domains make them well suited orchestrating cascade side. addition, it also discusses technical details approaches employed tackle mechanisms assemble function.