Hepatic Helicobacter species identified in bile and gallbladder tissue from chileans with chronic cholecystitis

摘要: Abstract Background & Aims: Cancer of the gallbladder is number one cause cancer mortality in Chilean women. Incidence rates for this tumor vary widely on a worldwide basis, being approximately 30 times higher high-risk than low-risk populations, suggesting that environmental factors such as infectious microorganisms, carcinogens, and nutrition play role its pathogenesis. Because several Helicobacter sp. colonize livers animals induce hepatitis, aim study was to determine whether infection associated with cholecystitis humans. Methods: Bile or resected tissue from 46 Chileans chronic undergoing cholecystectomy were cultured subjected polymerase chain reaction (PCR) analysis using -specific 16S ribosomal RNA primers. Results: Recovery frozen specimens unsuccessful. However, by PCR analysis, 13 23 bile samples 9 tissues positive . Eight amplicons sequenced phylogenetic analysis. Five sequences represented strains H. bilis , two " Flexispira rappini (ATCC 49317), strain pullorum Conclusions: These data support an association bile-resistant disease. Further studies are needed ascertain similar causative development cancer. GASTROENTEROLOGY 1998;114:755-763