Scanning electrochemical microscopy of membrane transport in the reverse imaging mode.

摘要: Scanning electrochemical microscopy (SECM), operated in reverse imaging mode (RIM), has been used to visualize the steady-state transport of molecules entering into porous membranes. RIM is advantageous for investigating across biological membranes situations where SECM tip can access only exterior membrane surface. Examples images a synthetic (mica with pores filled ion-selective polymer Nafion) and (hairless mouse skin) recorded during diffusive iontophoretic transport, are reported. allows visualization depletion solute solution adjacent pore openings. However, an accumulation above opening observed iontophoresis, which consequence separation from solvent (i.e., ultrafiltration). The results differences rates molecule transfer pore/solution interface when electroosmotic flow operative. suggest that may be useful measuring kinetics interfacial at