Targeting of hepatitis C virus core protein for MHC I or MHC II presentation does not enhance induction of immune responses to DNA vaccination.
作者： O. Vidalin , E. Tanaka , U. Spengler , C. Trepo , G. Inchauspe
关键词:
摘要: We analyzed different vaccine approaches aimed at enhancing CD4+- and CD8+-dependent responses against hepatitis C virus (HCV) core antigen. Specific DNA vectors expressing various forms of the in fusion with ubiquitin or lysosome-associated membrane protein (LAMP) were generated. These expressed full-length wildtype core; as a covalent ubiquitin; noncovalent containing N-stabilizing N-destabilizing residue; LAMP sequence. In vitro expression levels plasmids differed by much tenfold. After injection into mice, none yielded detectable antibody response, whereas core-specific cytotoxic T-lymphocyte (CTL) activity could be observed all long 21 weeks postimmunization. No increase CTL (ranging from 7% to 34% specific lysis) was ubiqu...
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