An epitope in hepatitis C virus core region recognized by cytotoxic T cells in mice and humans.
作者： M Shirai , H Okada , M Nishioka , T Akatsuka , C Wychowski
DOI: 10.1128/JVI.68.5.3334-3342.1994
关键词:
摘要: Several cytotoxic T-lymphocyte (CTL) epitopes have been defined in hepatitis C virus (HCV) proteins. CTL may play an important role the control of infection by HCV. Here, we identify a highly conserved antigenic site HCV core recognized both murine and human CTL. Spleen cells from mice immunized with recombinant vaccinia expressing gene were restimulated vitro 11 peptides protein. H-2d responded to single 16-residue synthetic peptide (HCV 129-144). This epitope was presented class I major histocompatibility molecule (H-2Dd) conventional CD4- CD8+ mapped using transfectants Dd, Ld, or Kd, but not seen restricted H-2b. The decapeptide LMGYIPLVGA. same two HCV-seropositive patients any seronegative donors. HLA-A2-positive acute chronic hepatitides 9-residue fragment (DLMGYIPLV) HLA-A2 containing HLA-A2-binding motif, extending only 1 residue beyond epitope. Therefore, this peptide, very prevalent HLA molecule, be valuable component vaccine against broad range isolates. study demonstrates that screening for prior useful.
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