Hormonal Mechanisms Regulating Hepatic Steroid Metabolizing Activities

摘要: The possible influence of androgens and estrogens on the sexual differentiation hepatic enzymes metabolizing corticosterone was studied in isolated perfused rat liver. The predominant metabolites livers from normal male rats were 3β,11β,21-trihydroxy-5α-pregnan-20-one, 5α-pregnane-3α,11β,20α, 21-tetrol 5α-pregnane-3β,11β,20β,21-tetrol excreted as mono- disulphates. Castration adult resulted a reduced formation 5α-pregnane-3,11β,20,21-tetrols decreased ratio between 20β 20α epimers. amount sulphate conjuged increased. Partial hepatectomy castrated followed by complete regeneration liver parenchyma 3α,11β,2 1-trihydroxy-5α-pregnan-20-one. Administration 100μg estradiol benzoate to slightly amounts 5αa-pregnane-3,11β,20,21-tetrol epimers formed, whereas same injection markedly (and 20β) oxidoreductase activity. The most pronounced effect administration seen subjected partial parenchymal under influence. In these metabolite profile almost that after perfusion female with corticosterone. 3αa,11β,15α,21-tetrahydroxy-5α,14ξ-pregnan-20-one, which has never been found rat. activities 3- 20-hydroxysteroid oxidoreductases those rats. Furthermore, major part monosulphates is typical rat. The present data show are capable shifting conjugation cells previously exposed towards pattern. However, an estrogen-mediated induction 15-hydroxylase active corticosterone, reaction liver, can only take place have not androgens.