Effect of alternative peritoneal dialysis solutions on cell viability, apoptosis/necrosis and cytokine expression in human monocytes1

摘要: Effect of alternative peritoneal dialysis solutions on cell viability, apoptosis/necrosis and cytokine expression in human monocytes. Background Cellular function, viability the network monocytes are influenced by specific composition (PD) fluids. In an vitro study using isolated blood monocytes, we investigated effect dialysates containing amino acids (Amino) or glucose polymer (Glu-poly) instead (Glu) as osmotic agent, bicarbonate (Bic) PBS lactate (Lac) a buffer. Methods The following parameters were studied: mitochondrial dehydrogenase activity (using MTT assay), interleukin (IL)-6 IL-8 release (ELISA) cellular IL-6 mRNA after lipopolysaccharide (LPS) stimulation RT-PCR). FACS flow cytometry with annexin V propidium iodide markers fluorescence microscopic methods used to effects test fluids necrosis apoptosis. Results Glu/Lac pH 5.5 Glu-poly/PBS 7.4 both significantly reduced more than 50% 60minutes incubation (30.5 ± 7.6%, 42.5 6.5%, referred RPMI 1640 100%). Amino/Bic Glu/Bic superior (MTT assay > 63%). rate necrotic cells 15minutes measured was mostly increased (29.9 4.0%). apoptotic cells, however, not different between solutions. concentration supernatant stimulated highest (1023 278pg/ml) (776 296pg/ml) lowest (46 22pg/ml) (32 13pg/ml). from showed similar pattern. suppressive (ratio IL-6/ β -Actin, 0.4 0.25 vs. 1.5 3.6). Conclusions Bicarbonate buffered agents when regarding metabolism, release, while some biocompatibility pattern for .