Online identification of the antioxidant constituents of traditional Chinese medicine formula Chaihu-Shu-Gan-San by LC–LTQ-Orbitrap mass spectrometry and microplate spectrophotometer

摘要: Chaihu-Shu-Gan-San (CSGS), a traditional Chinese medicine (TCM) formula containing seven herbal medicines, has been used in treatment of gastritis, peptic ulcer, irritable bowel syndrome and depression clinically. However, the chemical constituents CSGS had not studied so far. To quickly identify to understand profiles related antioxidant activity CSGS, liquid chromatography coupled with electrospray ionization hybrid linear trap quadrupole orbitrap (LC-LTQ-Orbitrap) mass spectrometry applied for online identification complex system, meanwhile, profile was investigated by fraction collecting microplate reading system. As result, 33 were identified. Among them, 13 components could be detected both positive negative ion modes, 20 determined only mode 2 mode. Meanwhile, potential also characterized combination 96-well plate collection elutes from HPLC analysis spectrophotometer, which scavenging activities free radical produced DPPH each directly reader. This study screened contribution fractions identified corresponding active constituents. The results indicated that LC-MS(n) assay system established this paper would useful strategy correlating TCMs their bioactivities without isolation purification.