Competition between SsrA tagging and translational termination at weak stop codons in Escherichia coli.
作者： Justine Collier , Emmanuelle Binet , Philippe Bouloc
DOI: 10.1046/J.1365-2958.2002.03045.X
关键词:
摘要: SsrA is a tmRNA involved in tagging polypeptides on stalled ribosomes. The resulting fusion proteins are then degraded. We purified endogenous SsrA-tagged by means of genetically engineered and identified some them. Analysis the suggested that they tagged at their C-terminal extremities. One them, ribokinase, expressed from messenger with poorly efficient stop codon, leading to translational recoding events. A change ribokinase coding sequence weak strong (UGAc UAAu) annihilated tagging. Translational termination UGA recruits release factor (RF) 2. observed was inversely correlated RF2 activity, revealing dynamic competition between
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