A preliminary study on the genetic profile of cag pathogenicity-island and other virulent gene loci of Helicobacter pylori strains from Turkey.

摘要: Abstract Helicobacter pylori genetic diversity affects the function and antigenicity of virulence factors associated with disease outcome. Gene profile was done to identify distribution gene loci within outside cag pathogenicity-island (PAI). H. strains from 35 patients [21 gastritis, 14 peptic ulcer diseases (PUD)] were analyzed using PCR. The PAI evaluated primers spanning 3′ end, cagA, promoter region cagE, cagT, 5′ end (LEC), extreme right plasticity open reading frames (ORFs), oipA (Hp0638) vacA alleles. We found few intact in examined. Deletions LEC1 (9.5% versus 14.3%), LEC2 (4.8% cagT (33.3% 28.6%), cagE (28.6% 28.6%) cagA (19.0% 42.9%) gastritis PUD strains, respectively. detectable 57.1% 92.9% PUD-associated strains. cagRJ also showed deletions for many its genes. detected 80.9% ORFs JHP912 JHP931 predominant vacA–s1a–m1a genotype PUD, while s2m2 This comprehensive analysis several genes PAI. However, oipA, JHP912, more than gastritis-associated suggesting importance on progression clinical