A novel sheathed surface organelle of the Helicobacter pylori cag type IV secretion system.
作者： Manfred Rohde , Jürgen Püls , Renate Buhrdorf , Wolfgang Fischer , Rainer Haas
DOI: 10.1046/J.1365-2958.2003.03549.X
关键词:
摘要: Type I strains of Helicobacter pylori (Hp) use a type IV secretion system (T4SS), encoded by the cag pathogenicity island (cag-PAI), to deliver bacterial protein CagA into eukaryotic cells and induce interleukin-8 secretion. Translocated is activated tyrosine phosphorylation involving Src-family kinases. The mechanism structural basis for not well understood. We describe here, confocal laser scanning microscopy field emission electron microscopy, novel filamentous surface organelle which part Hp T4SS. often located at one pole but can be induced cell contact also along lateral side bacteria. It consists rigid needle, covered focally or completely HP0527 (Cag7 CagY), VirB10-homologous protein. clustered in outer membrane. VirB7-homologous HP0532 found base this organelle. These observations demonstrate first time microscopic techniques complex T4SS-associated, sheathed reminiscent needle structures III systems.
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Steffen Backert, Stefan Moese, Matthias Selbach, Volker Brinkmann, Thomas F. Meyer, Phosphorylation of tyrosine 972 of the Helicobacter pylori CagA protein is essential for induction of a scattering phenotype in gastric epithelial cells. Molecular Microbiology. ,vol. 42, pp. 631- 644 ,(2008) , 10.1046/J.1365-2958.2001.02649.X
Renu B. Kumar, Yong-Hong Xie, Anath Das, Subcellular localization of the Agrobacterium tumefaciens T-DNA transport pore proteins: VirB8 is essential for the assembly of the transport pore. Molecular Microbiology. ,vol. 36, pp. 608- 617 ,(2002) , 10.1046/J.1365-2958.2000.01876.X
Jurgen Neesen, S. Padmanabhan, Hans Bunemann, Tandemly arranged repeats of a novel highly charged 16-amino-acid motif representing the major component of the sperm-tail-specific axoneme-associated protein family Dhmst101 form extended alpha-helical rods within the extremely elongated spermatozoa of Drosophila hydei. FEBS Journal. ,vol. 225, pp. 1089- 1095 ,(1994) , 10.1111/J.1432-1033.1994.1089B.X
Yuri Churin, Efterpi Kardalinou, Thomas F. Meyer, Michael Naumann, Pathogenicity island-dependent activation of Rho GTPases Rac1 and Cdc42 in Helicobacter pylori infection. Molecular Microbiology. ,vol. 40, pp. 815- 823 ,(2001) , 10.1046/J.1365-2958.2001.02443.X
E. Roine, W. Wei, J. Yuan, E.-L. Nurmiaho-Lassila, N. Kalkkinen, M. Romantschuk, S. Y. He, Hrp pilus: An hrp-dependent bacterial surface appendage produced by Pseudomonas syringae pv. tomato DC3000 Proceedings of the National Academy of Sciences of the United States of America. ,vol. 94, pp. 3459- 3464 ,(1997) , 10.1073/PNAS.94.7.3459
Stefan Odenbreit, Bettina Gebert, Jurgen Puls, Wolfgang Fischer, Rainer Haas, Interaction of Helicobacter pylori with professional phagocytes: role of the cag pathogenicity island and translocation, phosphorylation and processing of CagA. Cellular Microbiology. ,vol. 3, pp. 21- 31 ,(2001) , 10.1046/J.1462-5822.2001.00088.X
Rainer Haas, Chihiro Sasakawa, Hitomi Mimuro, Toshihiko Suzuki, Jiro Tanaka, Momoyo Asahi, Grb2 Is a Key Mediator of Helicobacter pylori CagA Protein Activities Molecular Cell. ,vol. 10, pp. 745- 755 ,(2002) , 10.1016/S1097-2765(02)00681-0
G. Liu, T. K. McDaniel, S. Falkow, S. Karlin, Sequence anomalies in the Cag7 gene of the Helicobacter pylori pathogenicity island Proceedings of the National Academy of Sciences of the United States of America. ,vol. 96, pp. 7011- 7016 ,(1999) , 10.1073/PNAS.96.12.7011
N. Nouwen, N. Ranson, H. Saibil, B. Wolpensinger, A. Engel, A. Ghazi, A. P. Pugsley, Secretin PulD: Association with pilot PulS, structure, and ion-conducting channel formation Proceedings of the National Academy of Sciences of the United States of America. ,vol. 96, pp. 8173- 8177 ,(1999) , 10.1073/PNAS.96.14.8173
Renu B. Kumar, Anath Das, Polar location and functional domains of the Agrobacterium tumefaciens DNA transfer protein VirD4 Molecular Microbiology. ,vol. 43, pp. 1523- 1532 ,(2002) , 10.1046/J.1365-2958.2002.02829.X