Induction of cell cycle arrest and apoptosis in JAR trophoblast by antimalarial drugs
作者： Athip NILKAEO , Suthinee BHUVANATH , Sakonwun PRAPUTBUT , Seuptrakool WISESSOMBAT
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摘要: Chloroquine, quinine, artemisinin, and pyrimethamine are generally considered safe drugs for treatment of malaria during pregnancy; however, high doses these detrimental with adverse outcome pregnancy. Since antimalarial interaction placental cells has not been addressed, in this study, we employed a non-radioactive proliferation assay lactate dehydrogenase (LDH) release assays to investigate the effect on JAR trophoblastic cell survival. All drug resulted inhibition dose-dependent fashion (p < 0.05) IC50 at 6.96, 6.49, 6.69, 6.89 microg/mL chloroquine, artemisinin pyrimethamine, respectively. In addition, was accompanied by increased cytotoxicity. Analysis progression cycle showed that triggered G0/G1 S phase arrest. Furthermore, induced apoptotic death as visualized DNA fragmentation analysis techniques. Findings study revealed cytotoxicity human trophoblast is mediated both arrest induction could have important implications use treating
sci-hub.st 参考文章(26)
E. Kerkhoff, E.B. Ziff, Cyclin D2 and Ha-Ras transformed rat embryo fibroblasts exhibit a novel deregulation of cell size control and early S phase arrest in low serum. The EMBO Journal. ,vol. 14, pp. 1892- 1903 ,(1995) , 10.1002/J.1460-2075.1995.TB07181.X
P. E. Duffy, M. Fried, Malaria in the pregnant woman. Current Topics in Microbiology and Immunology. ,vol. 295, pp. 169- 200 ,(2005) , 10.1007/3-540-29088-5_7
SoumaïIa Nabalma, Lady Khadiatou Traoré, Blaise Sondo, Innocent Pierre Guissou, Jean Testa, Chloroquine resistance of Plasmodium falciparum. Study of a surveillance method based on placental apposition and determination of blood chloroquine in pregnant women Cahiers d'études et de recherches francophones / Santé. ,vol. 8, pp. 293- 296 ,(1998)
Robert D. Newman, Monica E. Parise, Laurence Slutsker, Bernard Nahlen, Richard W. Steketee, Safety, efficacy and determinants of effectiveness of antimalarial drugs during pregnancy: implications for prevention programmes in Plasmodium falciparum-endemic sub-Saharan Africa. Tropical Medicine & International Health. ,vol. 8, pp. 488- 506 ,(2003) , 10.1046/J.1365-3156.2003.01066.X
Sornchai Looareesuwan, N.J. White, Juntra Karbwang, R.C. Turner, R.E. Phillips, Somboon Kietinun, Chloë Rackow, D.A. Warrell, QUININE AND SEVERE FALCIPARUM MALARIA IN LATE PREGNANCY The Lancet. ,vol. 326, pp. 4- 8 ,(1985) , 10.1016/S0140-6736(85)90056-X
Peter J. de Vries, Tran K. Dien, Clinical pharmacology and therapeutic potential of artemisinin and its derivatives in the treatment of malaria Drugs. ,vol. 52, pp. 818- 836 ,(1996) , 10.2165/00003495-199652060-00004
G.C. Cook, PREVENTION AND TREATMENT OF MALARIA The Lancet. ,vol. 331, pp. 32- 37 ,(1988) , 10.1016/S0140-6736(88)91012-4
Sanjeev Krishna, Nicholas J. White, Pharmacokinetics of quinine, chloroquine and amodiaquine. Clinical implications. Clinical Pharmacokinectics. ,vol. 30, pp. 263- 299 ,(1996) , 10.2165/00003088-199630040-00002
R. McGready, F. Nosten, The Thai-Burmese border: drug studies of Plasmodium falciparum in pregnancy. Annals of Tropical Medicine and Parasitology. ,vol. 93, pp. 19- 23 ,(1999) , 10.1080/00034989957709
Rose Mcgready, Kyaw Lay Thwai, Thein Cho, Samuel, Sornchai Looareesuwan, Nicholas J. White, François Nosten, The effects of quinine and chloroquine antimalarial treatments in the first trimester of pregnancy. Transactions of The Royal Society of Tropical Medicine and Hygiene. ,vol. 96, pp. 180- 184 ,(2002) , 10.1016/S0035-9203(02)90297-X