Assessing Pathogen Presence in an Intensively Tile Drained, Agricultural Watershed.

摘要: Increases in swine production and concomitant manure application provide beneficial nutrients for crops but also include the potential to spread pathogenic bacteria environment. While is known contain a variety of pathogens, little regarding long-term effect on fate transport this diverse set pathogens into surrounding waterways. We report use 16S-rRNA gene sequencing detect pathogen-containing genera agriculturally dominated South Fork Iowa River watershed, home approximately 840,000 76,000-ha basin. DNA was extracted from monthly grab samples collected three surface water sites two main artificial drainage outlets. sequences were matched with using targeted 16S rRNA amplicon sequencing. The specific quantified by combining percentage sequence matches quantitative polymerase chain reaction results. Specifically, abundances , significantly increased after typical fall application. Additionally, likely pathways identified. Surface concentrations influenced mainly drainage, whereas primarily transported waters runoff events. results study will help us understand environmental that may be useful mitigation agroecosystems capability alter existing microbial community structures.