Low-dose ionizing irradiation triggers a 53BP1 response to DNA double strand breaks in mouse spermatogonial stem cells.
作者： Wei Le , Lixin Qi , Jiaxuan Li , DengIong Wu , Jun Xu
DOI: 10.3109/19396368.2015.1125561
关键词:
摘要: The present study aims to examine the effect of low-dose ionizing irradiation on DNA double strand breaks (DSB) in mouse spermatogonial stem cells (SSCs) and reveal underlying pathways for repair DSB SSCs. Eighteen one-month-old mice were divided into 6 groups sacrificed separately at 45 minutes, 2 hours, 24 48 72 hours after 0.1Gy X-ray (mice without receiving served as control). After perfusion fixation, testes removed, sectioned, followed by staining γH2AX, 53BP1, Caspase 3, promyelocytic leukemia zinc-finger (PLZF) analysis among different groups. was observed immunofluorescence visualized confocal laser scanning. irradiation, only but not Caspase3 or γH2AX upregulated PLZF positive SSCs within minutes. expression level 53BP1 gradually decreased irradiation. Moreover, had no cell number apoptotic status However other spermatogenic highly expressed shortly which dramatically reduced following events repair. It appears that may cause cells. is involved Our data indicates plays an important role pathophysiological This open a new avenue understanding mechanisms male infertility.
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