Stable regenerants from long-term callus cultures of Ruscus hypophyllum L.

摘要: Plants were regenerated from callus derived stem explants of Ruscus hypophyllum L. The regenerating could be maintained by subculturing to basal medium containing BAP (0.5mg/l) for over five years. Detailed analysis mitotic and meiotic chromosomes phenotypes regenerants a period three years revealed no significant differences between cultured non-cultured plants. preliminary evidences indicate that genetic stability is in this species generated compact which provides with source rapid multiplication.