Factors determining the superior performance of lipid/DNA/protammine nanoparticles over lipoplexes.
作者： Giulio Caracciolo , Daniela Pozzi , Anna Laura Capriotti , Carlotta Marianecci , Maria Carafa
DOI: 10.1021/JM200237P
关键词:
摘要: The utility of using a protammine/DNA complex coated with lipid envelope made cationic 1,2-dioleoyl-3-trimethylammonium propane (DOTAP) for transfecting CHO (Chinese hamster ovary cells), HEK293 (human embryonic kidney NIH 3T3 (mouse embryonal and A17 (murine cancer cells) cells was examined. widely used DOTAP/DNA lipoplex employed as reference. In all the tested cell lines lipid/protamine/DNA (LPD) nanoparticles were more efficient in than lipoplexes even though composition same both devices. Physical-chemical properties found to control ability nanocarriers release DNA upon interaction cellular membranes. LPD complexes easily their payload, while remain largely intact accumulate at nucleus. Collectively, these data explain why often exhibit superior performances compared trasfecting represent promising class gene delivery.
acs.org
core.ac.uk
unicam.it
sci-hub.se 参考文章(67)
E. Marshall, BIOMEDICINE:Gene Therapy on Trial Science. ,vol. 288, pp. 951- 957 ,(2000) , 10.1126/SCIENCE.288.5468.951
E. Marshall, GENE THERAPY: What to Do When Clear Success Comes With an Unclear Risk? Science. ,vol. 298, pp. 510- 511 ,(2002) , 10.1126/SCIENCE.298.5593.510
Robert Pecora, Bruce J. Berne, Dynamic Light Scattering: With Applications to Chemistry, Biology, and Physics ,(1976)
Jun Yamauchi, Yasuhiro Hayashi, Kazuaki Kajimoto, Hidetaka Akita, Hideyoshi Harashima, Comparison between a multifunctional envelope-type nano device and lipoplex for delivery to the liver. Biological & Pharmaceutical Bulletin. ,vol. 33, pp. 926- 929 ,(2010) , 10.1248/BPB.33.926
Giulio Caracciolo, Daniela Pozzi, Augusto Amici, Heinz Amenitsch, Universality of DNA adsorption behavior on the cationic membranes of nanolipoplexes. Journal of Physical Chemistry B. ,vol. 114, pp. 2028- 2032 ,(2010) , 10.1021/JP9103382
S. Zuzzi, C. Cametti, G. Onori, S. Sennato, S. Tacchi, Polyion-induced cluster formation in different colloidal polyparticle aqueous suspensions. Langmuir. ,vol. 25, pp. 5910- 5917 ,(2009) , 10.1021/LA803844T
Hidetaka Akita, Asako Kudo, Arisa Minoura, Masaya Yamaguti, Ikramy A. Khalil, Rumiko Moriguchi, Tomoya Masuda, Radostin Danev, Kuniaki Nagayama, Kentaro Kogure, Multi-layered nanoparticles for penetrating the endosome and nuclear membrane via a step-wise membrane fusion process. Biomaterials. ,vol. 30, pp. 2940- 2949 ,(2009) , 10.1016/J.BIOMATERIALS.2009.02.009
S.-D. LI, L. HUANG, Surface-modified LPD nanoparticles for tumor targeting. Annals of the New York Academy of Sciences. ,vol. 1082, pp. 1- 8 ,(2006) , 10.1196/ANNALS.1348.001
Mohammad Ramezani, Majid Khoshhamdam, Ali Dehshahri, Bizhan Malaekeh-Nikouei, The influence of size, lipid composition and bilayer fluidity of cationic liposomes on the transfection efficiency of nanolipoplexes. Colloids and Surfaces B: Biointerfaces. ,vol. 72, pp. 1- 5 ,(2009) , 10.1016/J.COLSURFB.2009.03.018
Heinz Amenitsch, Giulio Caracciolo, Patrizia Foglia, Valentina Fuscoletti, Piero Giansanti, Carlotta Marianecci, Daniela Pozzi, Aldo Laganà, Existence of hybrid structures in cationic liposome/DNA complexes revealed by their interaction with plasma proteins. Colloids and Surfaces B: Biointerfaces. ,vol. 82, pp. 141- 146 ,(2011) , 10.1016/J.COLSURFB.2010.08.030