Cloning, functional expression, and developmental regulation of a neuropeptide Y receptor from Drosophila melanogaster.

摘要: Neuropeptide Y, peptide YY, and pancreatic polypeptide are homologous 36-amino acid peptides that differ from most other transmitters by having a relatively rigid conformation in aqueous solutions, defined as the fold, critical C-terminal tyrosine amide. These serve gastrointestinal hormones neurotransmitters. A cDNA encoding novel G protein-coupled receptor activated neuropeptide Y was cloned Drosophila use of degenerate oligonucleotide primers polymerase chain reaction amplification prepared transcripts expressed early embryogenesis. The encodes protein 449 amino acids with characteristics shares significant identity mammalian tachykinin receptors. When Xenopus oocytes, PR4 is neuropeptides order: YY greater than much polypeptide. Northern analysis showed at equivalent levels adult head body expression regulated during development. molecular characterization this should lead to better understanding functional role important family hormone receptors organisms