Revascularization of ischemic tissues with SIKVAV and neuropeptide Y (NPY).

摘要: Angiogenesis, the process of new vessel growth, is necessary for many normal physiological and pathological processes such as tumor wound healing ischemia. We have recently examined in vitroand vivothe ability two potent angiogenic compounds, SIKVAV (a peptide derived from alpha chain laminin-1) Neuropeptide Y (NPY) to revascularize ischemic tissue. These compounds were tested an ex vivo capillary sprouting angiogenesis assay that uses rat aortic rings. Both NPY presence VEGF, stimulated formation long sprouts at concentrations 1 ng (0.2 pmole/L) 100 μg SIKVAV. In comparison very little occurred control rings 50 VEGF alone was required induce equivalent number NPY. further a hindlimb model. (500 10 NPY) embedded hind limb following unilateral ligation femoral artery cm proximal adductor hiatus. After weeks peptides show or no revascularization distal ligation; however, both demonstrated two-fold increase vessels region ligation. Histological sections latex perfused ligated limbs had few latex-filled dermal capillaries. Limbs treated with NPY, distribution beds. data indicate are factors promising potential clinical application