Evaluation of primers and PCR performance on HPV DNA screening in normal and low grade abnormal cervical cells.

摘要: High risk human papillomaviruses (HR-HPVs) are associated with increased of normal cervical cells developing to dysplasia and carcinoma. Therefore, HR-HPV DNA testing can predict an endpoint carcinogenesis that is earlier than the development abnormalities. Not only sensitivity methods but also amount HPV very important might be parameters distinguish detection. In this study, we evaluated effects primer sets polymerase chain reaction (PCR) performance low viral load samples cytology (140 samples) mild using two consensus primers MY09/MY11 GP5+/6+. The PCR was performed single nested PCR. Positive both were then genotyped by dot blot hybridization. Results showed higher GP5+/GP6+ MY09/MY11. detected in 15% (21 140)and 20.7% (29 140) samples, respectively. For 37.1% (52 50% (70 GP5+/ GP6+. positivity rate 38.5% (54 GP5+/6+, 2 negative GP5+/6+ positive autonested These results suggested that, detection depends not on performance. 16 most common (20.8%), whereas type 58 found 11.1%. This study necessary for women participating cancer screening.