In situ transposon replacement and isolation of a spontaneous tandem genetic duplication.

摘要: Using a specialized transducing P1 phage carrying an insertion of Tn5-132, Tn5-wt in the chromosome Myxococcus xanthus, which codes for resistance to kanamycin, can be replaced with one tetracycline. That Tn5-132 daughter is inserted at same location as was parent shown by variety physical and genetic tests. Southern blot hybridizations restriction digests DNAs probed sequences homologous Tn5 show that same. When KmR transduced from TcR generalized myxophage Mx4 or Mx8, all transductants were TcS. Likewise, when used donor, its KmS. Flanking markers linked daughter. Spontaneous tandem duplications portions bacterial chromosomes trapped selectable marker donor recipient has different selecting both markers. Tc-replacement, this technique applied any region chromosome. We it isolate spontaneous duplication part M. xanthus The characterized Tn5-homologous DNA. It also unstable quantitation loss drug resistance. Transduction novel joint led reconstruction strain. All these tests gave results consistent proposed structure. methods described here are applicable bacterium into transposons introduced, some means exchange available.