Method of detecting mutations in DNA and RNA

摘要: A method of detecting a mutation specific nucleotide base in target nucleic acid chain comprises: (a) hybridizing labelled probe to the form hybrid which one end is positioned adjacent base; (b) adding derivative, e.g. thionucleotide, under conditions cause it join if complementary (c) digesting using an exonuclease enzyme such that derivative protects from digestion; and observing presence or absence label attached target. The can be used detect mutations even when these are not present at restriction cleavage sites, does require preliminary steps digestion, gel electrophoresis DNA (or RNA) blotting.