Fertilization and early embryology: Morphometric characterization of normal and abnormal human zygotes

摘要: Human zygotes (n = 278) from 96 in-vitro fertilization gonadotrophin-stimulated cycles were photographed in their pronuclear stage (16-18 h post-insemination). Normal morphological (two pronuclei) was observed 215 zygotes, 17 showed only one pronucleus, 40 three pronuclei and six four. Area, perimeter maximum minimum diameters of each zygote pronucleus measured using an IBAS 2000 (Kontron) image analyser. When the four groups compared, whole did not show any morphometric difference. However, these that a high number directly related to small pronuclei. Differences size linear increase nucleus/cytoplasm relationship found which varied with