Localization and synthesis of entactin in seminiferous tubules of the mouse.

摘要: Localization and synthesis of entactin in seminiferous tubules mouse testis was studied by immunocytochemistry. Frozen sections from adult mice testes were subjected to anti-entactin anti-laminin immunofluorescence. Both laminin localized within the tubule basement membrane intertubular region testis. The addition excess amount (but not fibronectin), premixed with antiserum, abolished immunostain. Western blotting showed that a protein extract preparation recognized antiserum comigrated recombinant entactin. Enriched fractions isolated primary Sertoli cells peritubular myoid cultured for 6 days on glass coverslip able synthesize secrete as detected immunofluorescence microscopy. Entactin also produced TM3 (Lcydig.Iike) TM4 (Sertoli-like) cell lines both blotting. distribution vs. differed. appeared mainly extracellularly. In contrast, intracellularly. above findings suggested I) existed testis, co-localized laminin; 2) synthesized lines; 3) exocytosed little intracellular accumulation, contrast an accumulation laminin.