Scaffold Function of Ca2+-Dependent Protein Kinase: Tobacco Ca2+-DEPENDENT PROTEIN KINASE1 Transfers 14-3-3 to the Substrate REPRESSION OF SHOOT GROWTH after Phosphorylation
作者： Takeshi Ito , Masaru Nakata , Jutarou Fukazawa , Sarahmi Ishida , Yohsuke Takahashi
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摘要: A molecular mechanism to ensure signaling specificity is a scaffold. REPRESSION OF SHOOT GROWTH (RSG) tobacco (Nicotiana tabacum) transcription factor that involved in gibberellin feedback regulation. The 14-3-3 proteins negatively regulate RSG by sequestering it the cytoplasm response gibberellins. N. tabacum Ca2+-dependent protein kinase NtCDPK1 was identified as an promotes binding of phosphorylation RSG. CDPKs are unique sensor responders Ca2+ only found plants and some protozoans. Here, we report scaffolding function CDPK. bound new mode. Autophosphorylation necessary for formation between but not its maintenance. formed heterotrimer with 14-3-3. Furthermore, transfers after dissociates from complex These results suggest interesting increases efficiency coupling catalysis on same protein.
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