Reduced Fat Mass in Rats Fed a High Oleic Acid–Rich Safflower Oil Diet Is Associated with Changes in Expression of Hepatic PPARα and Adipose SREBP-1c–Regulated Genes

摘要: PPARs and sterol regulatory element-binding protein-1c (SREPB-1c) are fatty acid-regulated transcription factors that control lipid metabolism at the level of gene expression. This study compared a high oleic acid-rich safflower oil (ORSO) diet high-butter for their effect on adipose mass expressions genes regulated by PPAR SREPB-1c in rats. Four groups Wistar rats were fed 30S (30% ORSO), 5S (5% 30B (29% butter + 1% or 5B (4% plus ORSO) diets 15 wk. Compared with group, group had less retroperitoneal white tissue (RWAT) lower mRNA lipoprotein lipase, adipocyte acid-binding protein, acid synthase, acetyl CoA carboxylase, SREBP-1c RWAT, higher acyl oxidase, carnitine palmitoyl-transferase 1A, binding mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase liver (P 2 fold those < 0.05). These results suggested smaller RWAT high-ORSO might be related to 18:2(n-6) 20:4(n-6). turn could upregulate catabolic through activation PPARalpha downregulate storage lipogenic suppression RWAT.