Only severe thrombocytopenia results in bleeding and defective thrombus formation in mice

摘要: Platelets are essential mediators of hemostasis and thrombosis. Platelet counts (PCs) in humans average 250 platelets/nL, but it is not entirely clear how platelet numbers affect occurrence thrombotic events. Mice, displaying PCs ~1000 widely used to assess function (patho-)physiology, also this species, the significance PC for disease established. We reduced mice defined ranges between 0 1000 platelets/nL by platelet-depleting antibodies challenged them different arterial thrombosis models: transient middle cerebral artery occlusion (tMCAO) stroke model tail bleeding experiments. show that injured aorta carotid were partially impaired when 70% or 80%, respectively. In contrast, times thrombus formation small arterioles largely unaffected reductions up 97.5%. Similarly, infarct growth neurological deficits after tMCAO 90%, whereas a further reduction was protective. These results reveal thrombosis, infarction, efficiently occur at unexpectedly low PCs, which may have implications risk hemorrhagic disease.