Intestinal anastomotic injury alters spatially defined microbiome composition and function

摘要: When diseased intestine (i.e., from colon cancer, diverticulitis) requires resection, its reconnection (termed anastomosis) can be complicated by non-healing of the newly joined resulting in spillage intestinal contents into abdominal cavity anastomotic leakage). While it is suspected that microbiota have capacity to both accelerate and complicate healing, associated genotypes functions not been characterized. Using 16S rRNA amplicon sequencing samples collected on day surgery (postoperative 0 (POD0)) 6th following (POD6)), we analyzed changes luminal versus tissue-associated at sites created rats. Results indicated injury induced significant with minimal differences microbiota. The most striking difference was a 500-fold 200-fold increase relative abundance Enterococcus Escherichia/Shigella, respectively. Functional profiling predicted predominance bacterial virulence-associated pathways post-anastomotic tissues, including production hemolysin, cytolethal toxins, fimbriae, invasins, cytotoxic necrotizing factors, coccolysin. Taken together, our results suggest compositional functional accompany tissues may potentially or healing.