Triarylmethane Fluorophores Resistant to Oxidative Photobluing

摘要: Spectral stability of small-molecule fluorescent probes is required for correct interpretation and reproducibility multicolor fluorescence imaging data, in particular under high (de)excitation light intensities super-resolution or single-molecule applications. We propose a synthetic approach to series spectrally stable rhodamine fluorophores based on sequential Ru- Cu-catalyzed transformations, evaluate their against photobleaching photoconversion the context other using chemometric analysis, demonstrate chemical reactivity fluorophore photoproducts. The substitution patterns providing photoconversion-resistant triarylmethane have been identified, applicability nonbluing labels live-cell STED nanoscopy demonstrated.