Solubilization of muscarinic acetylcholine receptor by zwitterionic detergent from rat brain cortex
作者： Moshe Gavish , Mordechai Sokolovsky
DOI: 10.1016/0006-291X(82)92013-7
关键词:
摘要: Abstract The muscarinic acetylcholine receptor was solubilized from rat brain cortex by zwitterionic detergent 3-[(3-chloramidopropyl) dimethylammonio]-1-propanesulfonate (CHAPS). About 15% of the binding activity and 40% destroyed detergent. Binding antagonist [ 3 H]-N-methyl-4-piperidyl benzilate (4NMPB) saturable. Scatchard analysis revealed a single population sites with K D value 0.7 nM B max 340 fmoles/mg protein. homogenate CHAPS treated pellet soluble receptors showed similar affinity for agonists oxotremorine carbamylcholine antagonists QNB atropine. dissociation 4NMPB appears slightly slower than membrane bound receptors.
elsevier.com
sci-hub.se 参考文章(20)
K.-P. Rueß, M. Liefländer, Action of detergents on covalently labelled, membrane bound muscarinic acetylcholinreceptor of bovine nucleus caudatus Biochemical and Biophysical Research Communications. ,vol. 88, pp. 627- 633 ,(1979) , 10.1016/0006-291X(79)92094-1
Tamas Bartfai, Jan Anner, Marianne Schultzberg, Johan Montelius, Partial purification and characterization of a muscarinic acetylcholine receptor from rat cerebral cortex Biochemical and Biophysical Research Communications. ,vol. 59, pp. 725- 733 ,(1974) , 10.1016/S0006-291X(74)80040-9
Orest Hurko, Specific [3H]quinuclidinyl benzilate binding activity in digitonin-solubilized preparations from bovine brain. Archives of Biochemistry and Biophysics. ,vol. 190, pp. 434- 445 ,(1978) , 10.1016/0003-9861(78)90296-5
Sofia Avissar, Yaacov Egozi, Mordechai Sokolovsky, Studies on muscarinic receptors in mouse and rat hypothalamus: a comparison of sex and cyclical differences. Neuroendocrinology. ,vol. 32, pp. 295- 302 ,(1981) , 10.1159/000123175
R.S. Aronstam, D.C. Schuessler, M.E. Eldefrawi, Solubilization of muscarinic acetylcholine receptors of bovine brain Life Sciences. ,vol. 23, pp. 1378- 1382 ,(1978) , 10.1016/0024-3205(78)90397-1
N. J. M. Birdsall, E. C. Hulme, BIOCHEMICAL STUDIES ON MUSCARINIC ACETYLCHOLINE RECEPTORS Journal of Neurochemistry. ,vol. 27, pp. 7- 16 ,(1976) , 10.1111/J.1471-4159.1976.TB01536.X
G. Scott Herron, Steven Miller, Wendy Lou Manley, Michael I. Schimerlik, Ligand interactions with the solubilized porcine atrial muscarinic receptor. Biochemistry. ,vol. 21, pp. 515- 520 ,(1982) , 10.1021/BI00532A016
H. Gorissen, G. Aerts, P. Laduron, Characterization of digitonin-solubilized muscarinic receptor from rat brain FEBS Letters. ,vol. 96, pp. 64- 68 ,(1978) , 10.1016/0014-5793(78)81063-1
Robert S. Aronstam, Leo G. Abood, Jesse Baumgold, Role of phospholipids in muscarinic binding by neural membranes. Biochemical Pharmacology. ,vol. 26, pp. 1689- 1695 ,(1977) , 10.1016/0006-2952(77)90146-0
Susan Carson, Cholate-salt solubilisation of bovine brain muscarinic receptors. Biochemical Pharmacology. ,vol. 31, pp. 1806- 1809 ,(1982) , 10.1016/0006-2952(82)90694-3