Production of polygalacturonase from Coriolus versicolor grown on tomato pomace and its chromatographic behaviour on immobilized metal chelates.

摘要: Tomato pomace and pectin were used as the sole carbon sources for production of polygalacturonase from a strain Coriolus versicolor in submerged culture. The culture C. grown on tomato exhibited peak activity (1,427 U/l) third day with specific 14.5 U/mg protein. by source increased time cultivation, reaching maximum 3,207 U/l fermentation broth 248 levels different isoenzymes produced during growth analysed native PAGE. Differential chromatographic behaviour lignocellulosic enzymes (i.e. polygalacturonase, xylanase laccase) was studied immobilized metal chelates. effect ligand concentration, pH, length spacer arm nature ion enzyme adsorption affinity chromatography (IMAC). these onto chelates pH-dependent since an increase protein observed pH 6.0 to 8.0. well other due coordination histidine residues which are available at surface presence imidazole equilibration buffer abolished A one-step purification devised using column Sepharose 6B-EPI 30-IDA-Cu(II) purified about 150 protein, final recovery 100% factor 10. use short higher selectivity this high factor. preparation SDS-PAGE "in situ" detection activity.