Inhibitory activities of selected Sudanese medicinal plants on Porphyromonas gingivalis and matrix metalloproteinase-9 and isolation of bioactive compounds from Combretum hartmannianum (Schweinf) bark

摘要: Periodontal diseases are one of the major health problems and among most important preventable global infectious diseases. Porphyromonas gingivalis is an anaerobic Gram-negative bacterium which has been strongly implicated in etiology periodontitis. Additionally, matrix metalloproteinases-9 (MMP-9) factor contributing to periodontal tissue destruction by a variety mechanisms. The purpose this study was evaluate selected Sudanese medicinal plants against P. bacteria their inhibitory activities on MMP-9. Sixty two methanolic 50% ethanolic extracts from 24 species were tested for antibacterial activity using microplate dilution assay method determine minimum concentration (MIC). seven methanol 62 MMP-9 determined Colorimetric Drug Discovery Kit. In search bioactive lead compounds, Combretum hartmannianum bark found be within active plant subjected various chromatographic (medium pressure liquid chromatography, column chromatography Sephadex LH-20, preparative high performance chromatography) spectroscopic methods (liquid chromatography-mass spectrometry, Nuclear Magnetic Resonance (NMR)) isolate characterize flavogalonic acid dilactone terchebulin as compounds. About 80% crude provided MIC value ≤4 mg/ml bacteria. revealed highest potency were: Terminalia laxiflora (wood; = 0.25 mg/ml) followed Acacia totrtilis (bark), Ambrosia maritima (aerial part), Argemone mexicana (seed), C. brownii (wood) extract T. (bark) with values 0.5 mg/ml. belong combretaceae family showed over at 10 μg/ml significantly inhibited IC50 6.7 μM. To best our knowledge, isolated hartmannianium first time study. Because some acting enzyme that would make them promising natural preference preventing treating