Phospholipid compositions of sera and synovial fluids from dog, human and horse: a comparison by 31P‐NMR and MALDI‐TOF MS

摘要: Alterations of the phospholipid (PL) compositions body fluids are assumed to be indicative inflammatory diseases, e.g. rheumatoid arthritis (RA). Recently, we have shown that particularly phosphatidylcholine/lysophosphatidylcholine (PC/LPC) ratio determined in human synovial (SF) and sera represents a reliable measure state RA patients. However, it is not yet clear what extent PC/LPC also affected by nutrition habits. In present study, PL corresponding acyl chain (SF serum patients as well from healthy volunteers) compared with those two other mammalian species (horses dogs suffering degenerative joint diseases controls) high-resolution 31P-nuclear magnetic resonance (NMR) spectroscopy matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). The most important result this study SF horse dog comparable fluids. Compared humans, however, fluid contains less PCs highly unsaturated arachidonoyl residues, while possesses highest content arachidonoyl-containing PC. These species-related differences stem primarily different habits (meat vs. plants).