Chlamydia trachomatis utilizes the host cell microtubule network during early events of infection

摘要: The host cell cytoskeleton is known to play a vital role in the life cycles of several pathogenic intracellular microorganisms by providing basis for successful invasion and promoting movement pathogen once inside cytoplasm. McCoy cells infected with Chlamydia trachomatis serovars E or L2 revealed, indirect immunofluorescence microscopy, collocation microtubules Chlamydia-containing vesicles during process migration from surface perinuclear location. vast majority microtubule-associated also collocated tyrosine-phosphorylated proteins. After migration, were positioned exactly at centre microtubule network, indicating microtubule-dependent mode chlamydial redistribution. Inhibition dynein, motor protein be involved directed vesicle transport along microtubules, was observed have pronounced effect on C. infectivity. Furthermore, dynein found collocate aggregates but not pneumoniae VR-1310, marked difference cytoskeletal requirements early infection events. In support this view, VR-1310 shown induce much less tyrosine phosphorylation HeLa proteins uptake than that seen trachomatis.