Bactericidal Effect of Bacteriocin of Lactobacillus plantarum K11 Isolated from Dongchimi on Escherichia coli O157

摘要: Among 68 strains of lactic acid bacteria (LAB) isolated from Dongchimi, a strain K11 was selected due to its bactericidal activity against Escherichia coli O157 The identified as Lactobacillus plantarum, based on physiological and biochemical characteristics. In the late exponential phase, La. plantarum showed maximum bacteriocin (12,800 BU/mL) maintained until early stationary phase. completely inactivated by all proteolytic enzymes such pepsin, protease, proteinase K, papain, chymotrypsin, trypsin, but not affected catalase, a-amylase, lysozyme, lipase, suggesting proteinaceous nature bacteriocin. Additionally, this in pH range 3.0 9.0 under storage conditions like 30 days at -20,4, or . Although absolutely lost after 15 min treatment 121, it relatively stable for 60 min. disappeared with acetone, benzene, ethanol, methanol, chloroform hexane. antibacterial good some LAB including spp., Enterococcus Streptococcus food-borne pathogens Bacillus Listeria Staphylococcus spp. well yeasts molds. Especially, intestinal Enterobacter aerogenes E. were significantly La, K11. Furthermore, addition 640 BU/mL resulted complete clearance 10 hr.