Development of an indirect ELISA for bovine mastitis using Sip protein of Streptococcus agalactiae.
作者： L. G. W. Xi , Z. Q. Shen , J. L. Wang , Y. Liu , C. DebRoy
关键词:
摘要: The sip gene encoding for a conserved highly immunogenic surface protein of Streptococcus agalactiae was amplified using polymerase chain reaction (PCR) and subcloned into prokaryotic expression vector pET32a (+) expressed as recombinant in E. coli BL21 (DE3). An indirect enzyme linked immunosorbent assay (ELISA) developed the purified Sip coating antigen, which could identify S. specific antibody sera. antigen at concentration 3.125 μg/ml, serum diluted to 1:160, HRP-conjugated secondary 1:4000 found be most effective exhibiting positive result. ELISA that may used detection pathogen mastitis cases, epidemiological studies surveillance.
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