Extra Pulmonary Tuberculosis: A Diagnostic Dilemma

摘要: Tuberculosis remains a major public health problem globally, with India being one of the high burden countries. The common causative agent is Mycobacterium tuberculosis but in developing countries M. bovis reported as potential human pathogen. Almost 20% all cases are extra pulmonary form disease. Diagnosis (EPTB) not always possible conventional methods, due to long time required and paucibacillary nature samples; hence need rapid molecular methods. A prospective study was conducted on 300 patients EPTB over period 5 years. These were suspected tubercular meningitis, ascites lymphadenitis. Samples analyzed cerebrospinal fluid, ascitic fluid lymph node fine needle aspirate. two step PCR targeting hup B gene used. Clinical response anti therapy (ATT) taken positive (gold standard). for 147 samples out 155 ATT responders. Of these 85.71% infected tuberculosis, 9.52% alone 4.76% showed co infection both M.tb bovis. sensitivity specificity 90.32 94.48% respectively.