Facile construction of a DNA tetrahedron in unconventional ladder-like arrangements at room temperature

摘要: A DNA tetrahedron as the most classical and simplest three-dimensional nanostructure has been widely utilized in biomedicine biosensing. However, existing assembly approaches usually require harsh thermal annealing conditions, involve formation of unwanted by-products, have poor size control. Herein, a facile strategy to fabricate discrete single, thermodynamically stable product quantitative yield at room temperature is reported. This system does not trigger or treatment initiate self-assembly. was made three chemically ligated triangular-shaped DNAs unconventional ladder-like arrangements, with measured heights ∼4.16 ± 0.04 nm, showing extra protections for enzymatic degradation biological environment. They show substantial cellular uptake different cell lines via temperature, energy-dependent clathrin-mediated endocytosis pathways. These characteristics allow our be used vehicles delivery very small temperature-sensitive cargos. novel developed tetrahedra could potentially extended other highly complex polyhedra; this indicated its generalizability.